DoCou Events

GoldenMutagenesis: GG Primer Design Workflow & Online Tool

by Chris Ulpinnis (ipb-halle), Pascal Puellmann (ipb-halle)

Europe/Berlin
Q-1-01 - Kurt Mothes Saal (KMS) (IPB Halle)

Q-1-01 - Kurt Mothes Saal (KMS)

IPB Halle

Weinberg 3, 06120 Halle
80
Description

This is a DoCou - IPB Doctoral Training Course. Please register.

Speakers: Chris Ulpinnis (IPB, Dept. SEB), Pascal Püllmann (IPB, UNG)

Description

Manuscript @ bioRxiv https://www.biorxiv.org/content/10.1101/453621v1

The course features a hands-on workshop on how to use the newly introduced platform “GoldenMutagenesis”. GoldenMutagenesis aims to simplify targeted specific and random mutagenesis based on the modular Golden Gate Cloning System.

Using real lab world examples the workshop covers in detail:

  • Upload of target sequence
  • Selection of mutagenesis type
  • Automated primer design for Golden Gate System
  • Detailed protocol for practical lab work
  • Automated analysis of mutant libraries (Quick Quality Control)
Organized by

Antje Hellmuth

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